Considerations Regarding the Antiinflammatory Action of Soy Total Extract Vs. Soy Isoflavonoid Daidzein on Animal Model

Țiulea Corina¹, Peev C¹, Feflea S¹, Dehelean C², Mureșan A³, Motoc A⁴

1 Department of Pharmacognosy, Faculty of Pharmacy, University of Medicine and Pharmacy Victor Babes, Timisoara
2 Department of Toxicology, Faculty of Pharmacy, University of Medicine and Pharmacy Victor Babes, Timisoara
3 Department of Morphopatology, Faculty of Medicine, University of Medicine and Pharmacy Victor Babes, Timisoara
4 Department of Anatomy, Faculty of Medicine, University of Medicine and Pharmacy Victor Babes, Timisoara

Introduction: The aim of this study is to investigate the anti-inflammatory action of daidzein on animal model a less studied isoflavone than genistein on this topic and to compare it’s anti-inflammatory activity with the one of soy total extract.
Material and method: Soybean seeds were grounded and a solvent formed of DMSO-ethanol-water in rapport 5-70-25 v/v/v was prepared. The extraction was made at room temperature using an ultrasonic bath (Falc LCD Series) for 30 minutes, 59 kHz. The solvent was evaporated with a rotary evaporator at 50 ºC. Daidzein was acquired from Extrasynthese (France).Animal studies were conducted on C57BL/6J female mice of 8 weeks. Edema was induced in both ears of each mouse by the topical application of 2 μg TPA dissolved in 20 μL of acetone to both the inner and outer ear surfaces. Thirty minutes after the application of TPA, the inner and outer surface of each ear was treated with 2 mg of soy total extract (group C) and 2 mg of daidzein (group D). Mice were killed after 24 h by cervical dislocation and ears were collected. Ears were weighed and histological analyze were made. For the histological analyze, tissue samples (skin) were fixed in 10% formalin solution and were embedded in paraffin and cut at 4 microns. Finally after deparaffinized the samples were stained with H&E (hematoxylin-eosin) and microscopically analyzed.
Results: The obtained results suggest that both of the samples used for the treatment are active agents against inflammation but with different empowering. Soy total extract is in charge of reducing the massive under epithelial inflammation with numerous granulocytes to a discrete under epithelial inflammatory infiltrate. Daidzein successfully reduced the massive under epithelial inflammation with numerous granulocytes to a discrete under epithelial edema.
Conclusion: Both daidzein and soy total extract present an anti-inflammatory action on animal model but daidzein is a better therapeutic choice since it has a stronger anti-inflammatory action.