Electrophoretic Separation of Proteins from the Drain Fluid by Geometric Electrofocusing in Conjunction with Local Complications in the Surgical Patient

Cozma D¹, Stoian M², Bacârea Anca³, Ieva Alina⁴, Iacob Oana³, Şchiopu A³, Copotoiu C¹

1 Surgical Clinic 1, County Emergency Clinical Hospital, Tîrgu Mureș, Romania
2 Clinic of Anesthesia and Intensive Care, County Emergency Clinical Hospital, Tîrgu Mureş, Romania
3 Department of Pathophysiology, University of Medicine and Pharmacy, Tîrgu Mureş, Romania
4 School of Doctoral Studies, University of Medicine and Pharmacy, Tîrgu Mureş, Romania

Introduction: Local postoperative complications are affecting the evolution of surgical patients, which is the main reason why early diagnosis is a priority concern. Our objective was to
Objective: To study the opportunity of protein analysis of the proteins from the drain fluid, as evolution markers of the surgical patient.
Material and method: We have analyzed drain fluid collected after 24 h, 72 h and 5 days after surgery. We have used the following: determination of protein concentration by spectrophotometric analysis at 280 nm and protein separation by geometric electrofocusing (patent no. 109585C1/30.03.1995)
Results: From the analyzed liquids, we obtained variable protein concentrations. In all cases, electrophoretic separation showed the presence of protein fractions similar to those of reference serum.
Conclusion: The analysis protocol allows precise quantitative determination of the proteins from the drain fluid. Geometric electrofocusing, approached for the first time for this specific type of analysis, has proved to be highly effective in terms of quality and affordable due to the low cost.