coordination | Acta Medica Marisiensis

Beneficial effects of metformin on haloperidol-induced motor deficits in rats. A behavioral assessment

George Jîtcă^1,2, Zsolt Gáll¹, Camil E Vari1, Bianca E Ősz¹, Amelia Tero-Vescan³, Alexandra Groșan¹, Maria T. Dogaru¹

1. Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Romania
2. Doctoral School of Medicine and Pharmacy, I.O.S.U.D., George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Romania
3. Department of Biochemistry, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Romania

DOI: 10.2478/amma-2021-0017

Objective: One of the most common side effects of haloperidol is the extrapyramidal syndrome, resulting from inhibition of nigrostriatal dopaminergic circuits and mitochondrial dysfunction due to structural similarities to pyridinium derivative, MPP+ that induce oxidative stress. In exchange, the use of metformin appears to enhance neurogenesis, energy metabolism, and oxidative status, so these properties can be speculated in the context of drug-induced pseudoparkinsonism by haloperidol.
Methods: To assess motor coordination and activity, rodents were divided into four groups: CTR (n = 10) – animals that received distilled water, METF (n = 10) – animals that received metformin 500 mg / kgbw, HAL (n = 10) – animals that received haloperidol 2mg / kgbw, HALMETF (n = 10) – haloperidol 2mg / kgbw and metformin 500 mg / kgbw. The treatment was administered for 34 days at the same time by gastric gavage, during which time behavioral tests, rotarod (days 7, 14, 21, 28), catalepsy (day 30), open field (day 32) and novel object recognition (day 34) were performed.
Results: The monitored parameters, showed significant differences between the groups of interest (HAL and HALMETF respectively), so that the administration of metformin at the beginning of treatment reduces the cataleptic behavior. The HALMETF group shows an attenuation of the motor deficit during the rotarod test and the freezing period from the Open Field test, is diminished.
Conclusions: Metformin treatment has a beneficial effect in haloperidol-treated rats, demonstrated by decreased cataleptic behavior, improved motor performance and reduced haloperidol-induced anxiety behavior.

Unsaturated Heteropolyoxotungstates with Platinum Cation Complexation

Grama Lavinia¹, Muntean Daniela-Lucia², Curticăpean A¹

1 Department of General and Inorganic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Tîrgu Mureș, Romania
2 Department of Analytical Chemistry and Drugs Analysis, Faculty of Pharmacy, University of Medicine and Pharmacy, Tîrgu Mureș, Romania

DOI: 10.2478/amma-2014-0007

Background: Polyanions are a special category of coordination compounds with a large development in last years. By coordination of metal oxoions at the lacunary polyoxometalates are obtained new compounds which are studied for theirs possible antitumoral and antiviral activities. The polyoxometalates can bind cations by oxygen atoms from their saturated surface structure or by embedding in vacant sites.
Material and method: The methods used for determining cation coordination with the unsaturated polyoxotungstate are spectrophotometry and conductometry. The solutions used in this study were: for ligand a solution of K27[KAsW40O140] and for cation a solution of K2[PtCl6].
The variation of electrical conductivity of ionic species found in solution, caused by their concentrations, decreases during the complex formation, which was determined by conductometry. The spectrophotometric assay was performed to verify ratios between cation:ligand combination, determined by conductometry.
Results: The graphical representations of conductivity function of number of moles of added titrant solutions emphasize that there are two types of coordination compounds with two different combination ratios ligand:cation at 1:2 or 1:4. The spectrophotometric determination performed, confirmed these ratios.
Conclusions: There are two types of coordination complexes, and the ligand:cation ratios are well known for encrypting polyoxotungstates type used in the study. Besides the main active position SC where K+ alkaline cation is coordinated, it has four active stand side S1–4, which can coordinate metal cations, depending on the size of their cationic radius and the electronic charge they hold.