Background: Over the last several decades allogeneic hematopoietic cell transplantation (HCT) has emerged as an important therapeutic option for a number of malignant and non-malignant conditions. The collection of hematopoietic stem cells mobilized from the bone marrow into the bloodstream of healthy donors has now become a routine procedure throughout the world.
Materials and methods: A number of 86 procedures of hematopoietic stem cells (HSC) harvest and cryopreservation from 64 volunteer donors, 54 adults (28 women and 26 men) and 10 children (5 girls and 5 boys) with ages between 6–66 years (on ave-rage 30.5) were carried out in the Bone Marrow Transplant Center from Clinical Institute Fundeni, Bucharest.
Results and discussions: HSC mobilization was achieved for all the 64 volunteer donors by administration of Filgastrim, on an average 8.4 mcg/donor weight (limits: 5–16.64 mcg/donor weight), leukapheresis procedure being realized in day +5 of Filgastrim administration.
Conclusions: In conclusion, a healthy volunteer donor, will undergo in most cases 4 or 5 days of Filgastrim administration. The WBC and the number of CD34+ cells from the periphe-ral blood will be counted beginning with the 4th day. When the number of CD34+ cells from peripheral blood will reach a certain level (usually on the 4th or 5th day), the vo-lunteer donor will be sent to the apheresis unit for harvesting stem cells.