plasma | Acta Medica Marisiensis

Rehabilitation in Patients with Anterior Cruciate Ligament Reconstruction Using Auxiliary Platelet-Rich Plasma Therapy

Albu Daniel – Emil¹, Copotoiu Monica², Melinte Răzvan¹, Bățagă Tiberiu¹, Copotoiu Sanda-Maria³

1 Department of Orthopedic Surgery and Traumatology I, County Emergency Clinical Hospital, Tirgu Mures, România
2 Department of Rheumatology and Physiotherapy, County Emergency Clinical Hospital, Tirgu Mures, România
3 Department of ICU and Anesthesiology I, County Emergency Clinical Hospital, Tirgu Mures, România

DOI: 10.1515/amma-2015-0122

Background: The main target after successful AnteriorCruciate Ligament (ACL) reconstruction is early rehabilitation. New options such as PRP (platelet rich plasma) may improve clinical outcomes. Objective: The aim of our study was to evaluate two consecutive series of patients who underwent ACL reconstruction, one with PRP treatment and one without it.
Material and method: Two groups of consecutive patients underwent arthroscopic ACL reconstruction, using the SemiT and BPTB techniques. Postoperatively all patients included in this study followed the same standardized rehabilitation protocol. In addition, patients in the first group received three intraarticular PRP injections as auxiliary therapy. Injections were performed at week two, four and six. The patients were evaluated at enrolment and every four and twelve weeks using the Tegner Lysholm Knee Scoring Scale (Scoring Scale: poor <65/ fair 65-83 / good 84-90 / excellent > 90). Each patient was operated on and evaluated afterwards by the same team of surgeons.
Results: At 12 weeks interval, Group A had a higher mean clinical score than Group B (94.67 vs 92.50) although marginally not statistically significant (p=0.0503, 95% CI:-4.336 to 0.002911). Regarding pain in patients from Group A compared with patients from Group B, we saw a statistically significant difference at 4 weeks interval (16.90 vs. 18.89, p=0.0370, 95% CI: 0.1260 to 3.842) and no significant difference at 12 weeks interval (21.19 vs. 21.94, p=0.3744, 95%CI: -0.9452 to 2.453). In terms of swelling points scored between the two groups, there was no statistically significant difference at 4 week interval (5.048 vs. 4.00, p=0.1979, 95% CI: -2.667 to 0.5714) but there is a significant difference in favor of patients from Group A at 12 weeks interval (8.475 vs. 5.556, p=0.0002, 95% CI: -4.323 to -1.159).
Conclusions: In the short term, the local treatment showed improvement on the overall clinical status of the patients (less pain, improved mobility, less swelling) undergoing rehabilitation after ACL reconstruction, although further studies are required.

Determination of Triiodothyronine and Thyroxine from Plasma and Milk of Lactating Cow

Nistor Cristina¹, Tero-Vescan Amelia¹, Ureche Ramona²

1 Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Tîrgu Mureş, Romania
2 Department of Hygiene, Faculty of Medicine, University of Medicine and Pharmacy, Tîrgu Mureş, Romania

Objective: The purpose of this study was to establish a method for the determination of T3 (triiodothyronine) and T4 (thyroxin) hormone concentrations in plasma, whole milk and after ethanol extraction, as well as to calculate the partition coefficient milk/plasma.
Methods: Ten Holstein Romanian friza milking cows were used to test the efficiency of the method. T3 and T4 were determined by an immunochemical ELISA competitive assay.
Results: Medium value of T3 in plasma was 2.78 ±1.53 ng/ml (4.27±2.35 nmol/l), in whole milk 3.72±1.44 ng/ml (5.71±2.21 nmol/l) and in extracted milk 4.97±1.67 ng/ml (7.63±2.56 nmol/l). Mean value of T4 in plasma was 50.97±7.30 ng/ml (65.60±9.39 nmol/l), in whole milk 2.12±0.87 ng/ml (2.73±1.12 nmol/l) and in extracted milk 3.60±1.15 ng/ml (4.64±1.48 nmol/l). Extraction from milk presented a good efficiency of 94.39% for T3 and 101.30% for T4.
Conclusion: The values obtained are in the concentration range reported by literature data for T4 and T3 from plasma and milk.