thin layer chromatography | Acta Medica Marisiensis

The Qualitative and Quantitative Determination of the Phenolic Compounds in Polygonum Convolvulus L. Species, Polygonaceae Family

Olaru OT¹, Anghel Adriana Iuliana¹, Istudor Viorica², Olaru Iulia Ioana³

1 Department of Pharmaceutical Botany and Plant Cell Biology, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
2 Department of Pharmacognosy, Phytochemistry and Phytotherapy, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
3 SC Dexter Invest SRL, Bucharest, Romania

DOI: 10.2478/amma-2013-0038

Introduction: Polygonum convolvulus L. (black bindweed), syn. Fallopia convolvulus (L.) Á. Löve, Polygonaceae family is a plant from the spontaneous flora, spread from the plain zone up to the subalpine zone. The objectives of our researches are the qualitative and quantitative determination of polyphenolic compounds from Polygoni convolvuli herba and the choice of the adequate solvent for obtaining an active pharmacological extract.
Method: The qualitative exam consisted of phytochemical screening and thin layer chromatography. The quantitative determination of the total polyphenols was made through the Folin-Ciocâlteu method.
Results: The flavonoids, the anthocyanins, the tannins and the phenol carboxylic acids (phytochemical screening) were emphasized and the following compounds were identified: rutin, hyperoside, isoquercitroside, quercetin, myricetin, kaempferol and caffeic acid.
Conclusions: In order to establish the technological lab process for obtaining an active pharmacological extract standardized in total polyphenols the adequate solvent is ethanol 50% (v/v).

Botanical Investigation Of Fallopia Dumetorum (L.) Holub (Polygonaceae) And Qualitative And Quantitative Assessment Of Its Polyphenolic Compounds

Olaru OT¹, Ancuceanu RV¹, Anghel Adriana Iuliana¹, Dinu Mihaela¹, Seremet Oana Cristina², Istudor Viorica³

1 Department of Pharmaceutical Botany and Cellular Biology, ”Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
2 Department of Pharmacology and Clinical Pharmacy, ”Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
3 Department of Pharmacognosy, Phytochemistry and Phytotherapy, ”Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania

DOI: 10.2478/amma-2014-0015

Background: Considering the continuous need to find new sources of polyphenolic compounds, we performed a pharmacognostical examination of the species Fallopia dumetorum (L.) Holub sin. Polygonum dumetorum L. (Polygonaceae). The plant is common in the plain regions of Romania and has not been exploited therapeutically.
Materials and method: Microscopic examination was performed on cross-sections, surface preparations and on powder obtained from the aerial parts of the flowering plant. Qualitative chemical analysis was realized by phytochemical screening and thin layer chromatography (TLC). Phenolic compounds were assayed by spectrophotometric methods: flavonoids expressed as rutin (with aluminium chloride), phenol-carboxylic acids expressed as chlorogenic acid (Arnow’s method) and proanthocyanidins expressed as cyanidin chloride (in acidic medium, by conversion to anthocyanins).
Results: The species has the following microscopic characters: anomocytic stomata, druses of calcium oxalate, sessile, pluricellular glandular hairs and pollen grains with smooth exine. Polysaccharides, reducing compounds, coumarins, sterols/triterpenes, phenol-carboxylic acids, flavones, proanthocyanidins, tannins and carotenoids were identified by phytochemical screening; chlorogenic acid, caffeic acid, quercetin and stigmasterol/beta-sitosterol were detected by TLC. F. dumetori herba has a content of 1.49 ± 0.105 g% polyphenol-carboxylic acids, 0.40 ± 0.087g% flavonoids and 0.18 ± 0.002 g% proanthocyanidins.
Conclusions: We have characterized pharmacognostically the native species F. dumentorum. Due to its content in phenolic compounds it might serve as a source of polyphenols.