Evaluation of Antimicrobial Effect of Mineral Trioxide Aggregate, Calcium Hydroxide and Calcium Hydroxide with Iodoform on Microbial Growth in Vitro

Background: The success of treatment of a young permanent tooth with pulp necrosis appropriate each stage of development depends primarily on the complete sterilization of infected root canals using specific endodontics drugs capable of destroying pathogenic microorganisms, especially obligate and facultative anaerobic bacteria.
Aim: The purpose of this research is to evaluate the antimicrobial effect of the three most used intraradicular substances, respectively mineral trioxide aggregate, calcium hydroxide and calcium hydroxide with iodoform on anaerobic bacteria isolated from infected root canals and also on pure bacterial species, namely Staphylococcus Aureus , Enterococcus Faecalis and Candida Albicans, cultures coming from the collection of the microbiology laboratory.
Material and methods: Anaerobic species grown on specific culture medium were prepared in the form of bacterial suspension using standardized system of 0, 5 McFarland turbidity diluted by half. The three drugs tested were used in different concentration and to each of them was added 1 ml of bacterial suspension obtained previously. From each sample obtained were made insemination on specific anaerobic medium and interpretation of results was made by comparing the number of bacterial colonies grown.
Results: Following this study MTA has failed to demonstrate superiority over calcium hydroxide in terms of antibacterial properties results showing that each substance tested has elective action on bacterial species.
Conclusion: All endodontic materials tested have shown antimicrobial properties able to effectively sterilize infected root canals but they compared different actions on bacterial species taken to study depending on the amount of active substance tested.

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