Tag Archives: Ki67

Preliminary Results on Clinico-pathological and Immunohistochemical Features of Malignant Melanomas

DOI: 10.2478/amma-2013-0047

Objective: To perform a retrospective clinico-pathological and immunohistochemical analysis of malignant melanomas.
Methods: One-hundred sixty consecutive cases diagnosed in the Department of Pathology of the County Emergency Clinical Hospital of Tîrgu Mureş, Romania, between January 2000 – December 2009, were used. Of these, 47 cases were melanoma metastases that were excluded from the study; this research was focused on the study of 113 primary melanomas (PMs). In 20 cases of PMs (11 cutaneous, 5 choroidal, and 4 melanomas of the anal canal) immunohistochemical stains were performed using the antibodies p53, Ki67, ER (estrogen receptor), CD8 and CD31.
Results: Cutaneous PMs prevailed (83%) followed by choroidal (11%), digestive tract (4%) and nasal mucosa PMs (2%). Independent of their location, PMs predominantly affected females (58%) over age 50. Regarding cutaneous cases, lower limbs (49%) were more affected, followed by the head and neck (18%) and the trunk (18%). We noted negativity or low p53-nuclear positivity in cutaneous and digestive tract PMs; p53 was overexpressed in choroidal melanomas. On the other hand, although the proliferative index Ki67 was also quite low in cutaneous PMs, its percentage increased in choroidal and digestive tract PMs. CD8 was positive in cutaneous PMs and negative in choroidal and digestive tract cases. While in cutaneous PMs we noted a predominated angiogenesis by intussusception, in digestive tract melanomas there was a predominance of sprouting and pseudoglomeruloid mechanisms.
Conclusions: Choroidal PMs display the highest p53 and Ki-67 expression, compared to cutaneous MMs. Tumor angiogenesis seems to present a specific pathway in cutaneous PMS compared to the gastrointestinal cases.

Full text: PDF

Immunoexpression of Ki67 and p53 in the Dental Follicles of Impacted Teeth

DOI: 10.1515/amma-2015-0033

Aim. The purpose of this study was to evaluate the morphological aspects of dental follicles associated with impacted teeth and the immunoexpression of Ki 67 and p53, in the remaining epithelium from impacted third molars and canines. Another aspect was to identify the transformation of the remaining epithelium within the odontogenic lesions.
Material and method. We included in the study a number of 100 dental follicles, of which only 62 met the inclusion criteria. The immunohistochemical reactions were carried out in the Immunohistochemistry Lab at the Department of Histology of the University of Medicine and Pharmacy of Târgu Mureş. We counted 100 cells from the remaining epithelial islands from which we determined the percentage of labeled cells for both markers. After this all data were subjected to a statistical analysis.
Results. Out of the 62 specimens 44 were from females and 28 from males. Positive results were obtained for the two markers but not for all cases. We have also found a positive correlation regarding the immunolabeling of the two markers (p=0.002 in canines, p=0.0001 in molars). Median values for ki67 were statistically significantly higher than the values for p53 for both types of dental follicles.
Conclusions. Both markers were expressed in the remaining epithelial islands. The stronger expression of Ki67 in the follicles of impacted molars can demonstrate the
higher frequency of odontogenic cysts in this area.

Full text: PDF